1329 Transglutaminase 2 deficiency leads to lipid accumulation and reduced autophagy in SZ95 sebocytes

Autophagy contributes to sebaceous gland function and the control of sebum composition. Based on literature data, autophagy markers were strongly expressed in maturing cells healthy skin, whereas downregulated acne-involved glands. Interestingly, tissue transglutaminase (TG2) which is known regulate mouse embryonic fibroblast has a potential role also integrity epidermis exerts an abundant expression In this work therefore, our aim was assess TG2 biology. Using knockout (KO) mice, water retention assay showed that repulsion higher KO mice compared wild-type animals. While Western blot high-content screening (HCS) results using permanent SZ95 sebocyte cell line generated with CRISPR/Cas9 method, absence sebocytes accumulated lipid droplets amounts, phenomenon associated lower levels such as ATG5, ATG7, LC3II/I. Our suggest may contribute production via regulation thus add TG2-related regulatory processes list possible targets for interventions dermatological disorders altered production.